Featured Protein Analysis by 2D-DIGE

Two Dimensional – Difference In Gel Electrophoresis (2D-DIGE) is a gel-based approach for comparative proteomics using fluorescent tags. Distinct fluorescent tags e.g.  Cy 3, 5 and 2 are used to label samples and a universal internal standard prior to 1st/2nd dimension electrophoresis. An automated software program is used to detect, quantify and annotate differentially expressed proteins. 2D-DIGE offers all the advantages of 2D-PAGE and overcomes the inherent disadvantage of variation and reproducibility problem in a 2D-PAGE.  

The scientists at ITSI-Biosciences had very early access to the 2D-DIGE technology in 2001; worked closely with Amersham Biosciences (now GE Healthcare) in the early days of commercialization in both promoting/validating the technology, and pioneering some key modifications in the workflow process e.g. recommending the switch to the Typhoon Digital Imager as the scanner for capturing 2D-DIGE images rather than the originally supplied scanner which bleached 2D-DIGE gels.

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Featured Mass Spectrometry

Liquid chromatography coupled to mass spectrometry (LC MS/MS) has become the backbone for understanding biochemical pathways to discovering new biomarkers for debilitating diseases. ITSI Biosciences has been utilizing advanced LC-MS/MS technologies to provide unsurpassed protein identification and characterization services for more than 6 years. The services are offered to both industrial and university clients at a competitive price and faster turnaround time.

We utilize state of the art techniques and constantly update our skills and equipment to accommodate the constantly evolving proteomics field.  Some of the areas of proteomics which we specialize in are listed below. This list is just for reference and is not exhaustive. Please don't hesitate to call us for techniques not listed here.

    1) In-gel-Digestion, 2)In solution/GelFree Digest, 3) Phosphopeptide Identification, 4)Biomarker Analysis (Plasma/Serum Depletion), 5)Quantification.

Services List

2d-dige services

Mass specTROMETRY services

SAMPLE PREP services

Multi-analyte analysis-XMAP

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