Protein Identification In-solution Digestion

In-solution / gelfree Digest
We offer in-solution digestion for un-fractionated or fractionated (i.e. HPLC or affinity purification) protein samples.  We offer services using traditional trypsin and other proteases including AspN, Glu-C, and ArgC. This approach eliminates time consuming gel electrophoresis and also reduces the loss associated with protein recovery during in-gel digestion. We have expertise to optimize nano-LCMS for working with tryptic peptides and also with non-tryptic peptides including those naturally occurring – (e.g. from cerebrospinal fluid).

Our peptide sequencing and protein identification by tandem mass spectrometry services includes:

For a 90 Minute Run (Cat# S-0031.2)

a) In-solution digestion including reduction ad alkylation
b) Tandem mass spectrometry analysis using nano-LC/MS/MS,
c) Database search,
d) Full protein annotation including GO annotation.
Cost is per sample.

    For a 180 - 240 Minute Run (Cat# S-0031.3)

    a) In-solution digestion including reduction ad alkylation
    b) Tandem mass spectrometry analysis using nano-LC/MS/MS,
    c) Database search,
    d) Full protein annotation including GO annotations.
    Cost is per sample.

    Contact Us for more information on this service.

 

 


Important Info

  • Project Summary FormClick here to download.
  • Frequently Asked Questions Have a questions? Click here for FAQs on LC/MS/MS