Protein Isolation Kit for Two-Dimensional Difference Gel Electrophoresis (ToPI-DIGE, K-0010)
$76.62 – $81.44
ToPI-DIGE is optimized for isolation of total proteins from cell lines (K-0010-C) and mammalian tissue (K-0010-T) for 2-Dimensional difference in gel-electrophoresis (2D-DIGE).
The ToPI-DIGE™ Kit contains a set of optimized reagents and standard operating procedures that allow basic and clinical research scientists to easily, conveniently and reproducibly isolate total proteins from human and non-human cell lines (attached and suspended), solid tissue and tumor biopsies for use in 2D-DIGE experiments (Fig 1).
ToPI-DIGE is optimized for isolation of total proteins from cell lines (K-0010-C) and mammalian tissue (K-0010-T) for 2-Dimensional difference in gel-electrophoresis (2D-DIGE). Using the kit prevents protein breakdown and prevents additional buffer exchange steps that may lead to protein breakdown and lower yield.
(Protease-, RNase- & DNAse- free)
Fig 1: 2D-DIGE image of Human Breast Cancer Cell Lines Proteins were isolated with ToPI-DIGE Kit. 50ug of protein from “reference”, “test” and “Internal Standards” were labeled with Cyanine dyes and loaded on the gel.
Each Kit contains optimized buffer systems and consumables including:
- Buffer 1
- Buffer 2
- Buffer 3
- iTube A
- iTube B (Protease-, RNase- & DNAse- free)
- Validated SOP for total protein isolation from cell lines and tissue for 2D-DIGE
Additional information
| Weight | 2 lbs |
|---|---|
| Dimensions | 12 × 9 × 2 in |
| Type | Cell Lines (10 Assays), Mammalian Tissue (10 Assays) |
Data Sheet(s)
Click on the title(s) below to view the product data sheet.
Citations
Acute mitochondrial dysfunction after blast exposure: potential role of mitochondrial glutamate oxaloacetate transaminase.
P. Arun, R. Abu-Taleb, S. Oguntayo, Y. Wang, M. Valiyaveettil, J. Long, M.P. Nambiar Journal of Neurotrauma
Article Snippet: “Proteomic analysis of brain cortex was carried out as described by us earlier. Briefly, proteins were isolated from the brain cortex of sham control and repeated blast exposed mice (3 animals/group) at 6 h post-blast using the ToPI-DIGE™ total protein isolation kit (ITSI-Biosciences, Johnstown, PA) and subjected to two-dimensional differential in-gel electrophoresis (2D-DIGE).”
The effect of selenium enrichment on baker’s yeast proteome.
K. El-Bayoumy, A. Dasa, S. Russell, S. Wolfe, R. Jordan, K. Renganathan, T.P. Loughranb, R. Somiari. Journal of Proteomics-Elsevier Inc. 2011 October 21;.10.1016
Article Snippet: “Approximately 150 mg dry weight of SEY and RY were transferred to separate tubes and suspended in 1ml ToPI-DIGE™ Buffer – 2 (ITSI – Biosciences, Johnstown, PA) consisting of 7 M urea, 2 M thiourea, 4% CHAPS, 0.5% NP-40, 5 mM magnesium acetate, 30 mM Tris-HCl, pH 8.5, and disrupted using a polytron homogenizer as previously described [20].“
Proteomics of rat prostate lobes treated with 2-N-Hydroxylamino-1-methyl-6-phenylimidazo [4,5-b] pyridine, 5alpha-dihydrotestosterone, individually and in combination.
Boyiri, T., Somiari, RI., Russell, S., EL-Bayoumy, K. Int. J Oncol. 2009 Sep;35(3):559-67
Article Snippet: “Proteins were isolated from the frozen prostate lobes using the ToPI-DIGE™ total protein isolation kit (ITSI-Biosciences, Johnstown, PA). Briefly, each frozen lobe was rapidly homogenized in about 50 μl of ToPI Buffer-2 (7 M urea, 2 M thiourea, 4% Chaps, 0.5% NP-40, 5 mM magnesium acetate, 30 mM Tris-HCl, pH 8.5) using clean disposable plastic pestles supplied with the kit.”
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