Tandem Mass Spectrometry Services (LC-MS/MS)
ITSIBIO »Our Tandem Mass Spectrometry Services (LC-MS/MS) provide highly sensitive and specific analysis of small molecules and proteins, allowing for accurate detection and identification in complex samples.
Reach out to us for more information. Also, view our list of citations involving our LC-MS-MS services or read more about the services we offer below.
Sample Preparation
Our Mass Spec Services include sample preparation, ensuring accurate and reliable analysis of complex biological samples for research and clinical applications. We have over 11 sample preparation procedures.
Reach out to us for more details.
Proteomics Service
Our Proteomics Service facilitates the study of proteins and their functions, aiding in the development of personalized medicine and the discovery of new drug targets for various diseases.
xMAP Analysis
We provide xMAP analysis services that offer simultaneous detection and quantification of multiple analytes in a single sample, providing high-throughput analysis and enabling efficient screening of potential biomarkers for various diseases.
Metabolomics Service
By enabling the in-depth analysis of small molecules, our Metabolomics Service offers valuable insights into metabolic pathways and the identification of disease biomarkers. This is vital for disease diagnosis, treatment, and monitoring.
Protein Identification
We offer a specialized service that provides accurate and dependable protein sequencing and identification, which plays a critical role in comprehending protein function and discovering novel therapeutics and diagnostics for multiple diseases.
And More…
Apart from the above-mentioned analytical services, we have a range of services that utilize Tandem Mass Spectrometry technology to derive test results. Join us today and access cutting-edge technology. Contact us for more details.
| S/N | CAT NO. | NAME | DESCRIPTION | UNIT COST |
|---|---|---|---|---|
| 1 | SP1 | Sample Preparation 1 | Homogenization, protein quantitation using ToPA Bradford protein assay kit. | Per sample |
| 2 | SP2 | Sample preparation 2 | Includes, precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA. | Per sample |
| 3 | SP3 | Sample preparation 3 | Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Column kit. | Per sample |
| 4 | SP4 | Sample preparation 4 | Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Solvent kit. | Per sample |
| 5 | SP5 | Sample preparation 5 | Depletion IgY Column Depletion of 14 abundant proteins from serum / plasma using IgY column. | Per sample |
| 6 | SP6 | Sample preparation 6 | Phospho-protein/ peptide enrichment using TiO2 or IMAC technology. | Per sample |
| 7 | SP7 | Sample preparation 7 | In-gel digestion. Digestion of proteins in a gel band/spot using trypsin. The service includes a) gel de-staining – coomassie or silver stain b) alkylation and reduction c) trypsin digestion d) peptide extraction from the gels for mass spectrometry analysis. | Per sample |
| 8 | SP8 | Sample preparation 8 | In-solution digestion. Digestion of proteins in solution using trypsin. The service includes; a) determining protein concentration (if possible), b) alkylation and reduction c) digestion d) peptide extraction for mass spectrometry analysis. | Per sample |
| 9 | SP9 | Sample preparation 9 | Urine protein isolation and concentration. Service includes Isolation, partial purification, and concentration of urine proteins using ITSI Urine Protein Isolation kit. | Per sample |
| 10 | SP10 | Sample preparation 10 | Zip tip cleanup of in-gel or in-solution digested samples…. | Per sample |
| 11 | SP11 | Sample preparation 11 | Any other sample preparation. | Per sample |
| 12 | S50.FPS | Flash Proteomics Service (FPS) by tandem mass spectrometry | The Flash Proteomics Service involves the use of validated pre-fractionation protocols and high performance tandem mass spectrometers to rapidly generate a global qualitative protein signature of a moderately-complex specimen. With FPS the run time is 30 minutes or less and most results will be ready in about 48h. Samples including fractions from HPLC, pre-fractionated samples such as protein lysates, serum, plasma and culture media can be screened. In many cases, results will be ready in 48 hours. This service is ideal for preliminary data generation, to support grant applications, biomarker research, search for contaminants in samples, verification of the integrity and purity of samples, characterization of antibodies and recombinant proteins. The standard FPS service includes: • Sample preparation, • Nano-LC/MS/MS. • Database search and identified protein annotation. | Per sample |
| 13 | S50.60 | LC60-MS/MS | Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of pure protein samples. Services include • Tandem mass spectrometry analysis using nano-LC/MS/MS, • Database search and • Identified protein annotation – 60 min run. | Per sample |
| 14 | S50.90 | LC90-MS/MS | Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of slightly complex samples, including proteins isolated from gel plugs and gel bands. Services include • Tandem mass spectrometry analysis using nano-LC/MS/MS, • Database search and • Identified protein annotation- 90 min run. | Per sample |
| 15 | S50.120 | LC120-MS/MS | Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of moderately complex samples. Services include • Tandem mass spectrometry analysis using nano-LC/MS/MS, • Database search and • Identified protein annotation – 120 min run. | Per sample |
| 16 | S50.240 | LC240-MS/MS | Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of very complex samples. Services include • Tandem mass spectrometry analysis using nano-LC/MS/MS, • Database search and • Identified protein annotation – 240 min run. | Per sample |
| 17 | S60 | LC-MS/MS-PTM | Peptide sequencing and Post Translational Modification (PTM) Mapping of purified or partially purified proteins by Mass Spectrometry. Services include • Tandem mass spectrometry analysis using nano-LC/MS/MS, • Database search • PTM Mapping and • Identified protein annotation. | Per sample |
| 18 | S70 | MudPIT Proteomics. | Multi-dimensional Protein Identification Technology and peptide sequencing. Services include • Off line SCX fractionation into multiple fractions, • Online Reverse Phase (RP) liquid chromatography (LC) – tandem mass spectrometry analysis using nano- LC/MS/MS, • Database search and • Identified full protein annotation. | Per sample |
| 19 | S80 | iTRAQ based Proteomics. | Quantitative proteomics using Isobaric Tags for Relative and Absolute Quantitation (iTRAQ). Labeling with 2-8 tags can be used. iTRAQ Labeling is a non-gel based protein quantitation technique using 5 – 100 µg of protein. 2 to 8 samples can be compared in a single experiment. Services include • In-solution digestion • iTRAQ labeling • SCX cleanup, • Tandem mass spectrometry analysis using nano- LC/MS/MS, • Database search and • Identified full protein annotation. | 2-8 samples |
| 20 | S81 | iTRAQ-MudPIT Proteomics. | iTRAQ plus MudPIT. Service includes • Multi-dimensional offline fractionation of samples into 2-20 fractions, • LC-MS/MS analysis of all fractions, • Database search and annotation of identified proteins. | Per sample |
| 21 | S90 | TMT based Proteomics. | Quantitative proteomics using Tandem Mass Tags (TMT). Labeling with 2-8 tags can be used. TMT Labeling is a non-gel based protein quantitation technique using 5 – 100 µg of protein. 2 to 8 samples can be compared in a single experiment. Services include • In-solution digestion • TMT labeling • SCX cleanup, • Tandem mass spectrometry analysis using nano- LC/MS/MS, • Database search and • Identified full protein annotation. | 2-8 sample |
| 22 | S91 | TMT-MudPIT Proteomics. | TMT plus MudPIT. Service includes • Multi-dimensional offline fractionation of samples into 2-20 fractions, • LC-MS/MS analysis of all fractions, • Database search and annotation of identified proteins. | Per sample |
| 23 | S100 | xMAP analysis | Quantitative protein analysis using Luminex xMAP technology. The level of up to 100 proteins can be measured simultaneously in 50ul of serum, plasma, tissue lysate and other body fluids. | 1-100 samples |
| 24 | S110 | Metabolomics – PMP | Primary Metabolism Profiling (PMP): This analysis targets carbohydrates and sugar phosphates, amino acids, hydroxyl acids, free fatty acids, purines, pyrimidines, aromatics, exposome-derived chemicals. | Per Sample (minimum 12 samples) |
| 25 | S111 | Metabolomics – CLP | Complex Lipids Profiling (CLP): This analysis will target Ceramides, Sphingomyelins, Cholesteryl esters, Oxysterols, Lyso- and phospholipids, mono-, di- and triacylglycerols, galactosyl- and glucuronyllipids. | Per Sample (minimum 12 samples) |
| 26 | S112 | Metabolomics – BAP | Biogenic Amines Profiling (BAP): This analysis will target acylcarnitines, TMAO, cholines, betaines, SAM, SAH, nucleotides and nucleosides, methylated and acetylated amines, di- and oligopeptides. | Per Sample (minimum 12 samples) |
| 27 | S113 | Metabolomics – TMA | Targeted Metabolite Analyses (TMA): This analysis measures bile acids, steroids, eicososanoids / oxylipins and, very short chain fatty acids using liquid chromatography-QTRAP or gas chromatography-quadrupole mass spectrometry | Per Sample (minimum 12 samples) |
| 28 | DBS-PID | Database Search – Protein Identification | Database search for protein identification. The service includes • Downloading the most recent database files from NCBI or other relevant sources • Database search and • Identified protein annotation. | Per search |
| 29 | DBS-PTM | Database Search – Protein Identification and PTM Mapping | Database search for protein identification and post translational modification mapping. The service includes • Downloading the most recent database files from NCBI or other relevant sources • Database search and • Identified protein annotation. | Per search |
| 30 | GO | Gene Ontology (GO) Annotation | Annotation of proteins using GO classification for biological process, molecular function and cellular component. | Per search |
| 31 | MSI | Manual Spectrum Interpretation | Manual Interpretation of mass spectrum | Per hour (Minimum one hour) |
Citations
Browse through a list of scientific publications and research papers involving our LC/MS/MS Services.
Perturbation of Iron Metabolism by Cisplatin through Inhibition of Iron Regulatory Protein 2.
Miyazawa, Masaki 2, Alexander R. Bogdan, Yoshiaki Tsuji 3. Cell Chemical Biology, November 15, 2018, Volume 26, Issue 1, 85 – 97.e4. doi: 10.1016/j.chembiol.2018.10.009
Identification of STAT2 serine 287 as a novel regulatory phosphorylation site in type I interferon-induced cellular responses.
H. C. Steen, S. Nogusa, R. J. Thapa, S.H. Basagoudanavar, A. Gill, S. Merali, C. A. Barrero, S. Balachandran, A. M. Gamero, The Journal of Biological Chemistry
Senescence Marker Protein 30 (SMP30) Expression in Eukaryotic Cells: Existence of Multiple Species and Membrane Localization.
Peethambaran Arun, Vineela Aleti, Kalpana Parikh, Veeraswamy Manne, Nageswararao Chilukuri. PLos ONE. 2011 February; Volume 6, Issue 2
Down-regulation of 14-3-3 isoforms and annexin A5 proteins in lung adenocarcinoma induced by the tobacco-specific nitrosamine NNK in the A/J mouse revealed by proteomic analysis.
Bortner JD Jr, Das A, Umstead TM, Freeman WM, Somiari R, Aliaga C, Phelps DS, El-Bayoumy K. J Proteome Res. 2009 Aug;8(8):4050-61. doi: 10.1021/pr900406g
