$33.75 – $4,995.00
RNAssure provides robust, reliable RNA protection against all RNases as well as exposure to oxidation. Maximum RNA protection is achieved by treating purified RNA samples with RNAssure as soon as they are isolated. With RNAssure, RNA can be protected even at room temperature, eliminating the concern that RNA may degrade during routine experimental protocols or due to catastrophic freezer failure. RNAssure easily integrates into extraction and purification kits from all major life science manufacturers. RNAssure protected samples can be used directly in downstream applications without the need for further purification.
Maximum RNA protection is achieved by treating purified RNA samples with GenTegra RNAssure as soon as they are isolated. RNAssure is a robust, reliable protection product that utilizes GenTegra’s proven, patented Active Chemical Protection™ (ACP) chemistry to protect RNA against all RNases as well as exposure to oxidation. RNAssure eliminates the potential that RNA may degrade during every day experimental protocols or due to catastrophic freezer failure. RNAssure can protect RNA for at least 3 days at room temperature (RT) and 2 weeks at 4 °C. It easily integrates into extraction and purification kits from all major life science manufacturers including, for example, Invitrogen, Zymo, and QIAGEN. GenTegra RNAssure also protects RNA from damage during DNase treatment at the higher temperature of 37C..
- Inactivate all residual RNase activity carried over from purification process or environmental contamination
- Protect RNA from oxidation damage, non-specific adherence to plastic, and adverse or accidental exposure to higher temperatures
- Allow RT handling of RNA samples and 4°C storage for weeks without losing RNA integrity
- Protect RNA during DNase treatment when exposed to RT or 37°C for extended periods of time
- Integrates seamlessly into standard RNA extraction kit workflows
RNAssure easily integrates with your standard RNA purification workflow
RNAssure Elution Tubes replace the manufacturer’s supplied elution collection tubes and integrates seamlessly with standard column-based purification kits and protocols.
RNA is protected from all trace RNases with RNAssure
The amount of RNase contamination varies widely depending on sample types and extraction methods, and it is extremely difficult to assess the level of contamination in an extracted RNA sample. RNAssure increases RNA stability in the liquid state at 37 °C even in the presence of trace amounts of RNase.
RNA integrity is maintained in the liquid state in the presence of increasing amounts of RNase A (left) and RNase I (right) only when protected with GenTegra RNAssure.
RNAssure protects RNA from degradation even at very short exposures to RT
Keeping RNA samples on ice slows degradation. However, during normal experimental handling, the sample temperature can often rise above 0 °C. Even brief exposure to elevated temperature is detrimental to RNA, especially with prevalent contamination from endogenous or environmental RNases1,2.
Within less than 15 minutes of exposure at RT, unprotected RNA shows noticeable degradation with progressive deterioration of the RNA quality over longer exposure times.
RNAssure protected RNA shows no significant change in RNA quality throughout the 4-hour exposure to RT.
RNAssure protects RNA from degradation for long-term exposure at RT
In the presence of RNAssure, no significant degradation was observed compared to control RNA for a minimum of 3 days and up to 7 days at RT.
Following 3 or 7 days of incubation at RT, commercially sourced purified mouse spleen RNA samples were analyzed on a Bioanalyzer for both fragmentation length and RINe scores. A. Prior to incubation at RT, control mouse spleen RNA showed no significant degradation. B. After 7 days at 25 °C, RNA showed severe degradation in the absence of RNAssure. C. Whereas, in the presence of RNAssure, no significant degradation was observed compared to control RNA. D. At 25 °C, it is observed that RIN values for the unprotected RNA (orange) showed a dramatic decrease at 3 days, while the RNAssure protected RNA (green) maintained high RINe scores even after 7 days.
RNAssure protects RNA from degradation during DNase treatment at 37°C
The presence of RNAssure provides added protection against damage to the RNA during the DNase treatment at elevated temperature.
RNAssure protects RNA during DNase Treatment. RNA was extracted from various mouse organs using either Trizol-based method or a leading commercial column-based kit A. While mouse liver RNA extracted by Trizol was free of gDNA contamination, column-based kit led to significant contamination of gDNA of various sizes (red arrows). B. RNA extracted from brain, intestine, lung, and kidney, using column-based method, was subjected to in-solution DNase treatment following manufacturer’s instruction (37°C for 30 min). After treatment, RNA from different samples showed moderate to severe degradation, evidenced by decreased 18S rRNA (top band) vs 28S rRNA (bottom band). In contrast, RNAssure helped significantly to maintain the RNA integrity during DNase digestion.