Autoantibody Detection Kit (K-0046)

Autoantibodies are a type of antibodies that are produced against substances formed by a person’s own body. Autoantibodies can directly destroy cells that have the substances on them, or they can make it easier for white blood cells to recognize and eliminate the substance¹. Many individuals have autoantibodies, and it is known that some autoimmune diseases are caused by autoantibodies.

The production of autoantibodies may be due to genetic predisposition, hormones, therapeutic drugs or an environmental component including exposure to viruses and toxic chemicals. The presence of autoantibodies is associated with autoimmune disorders and literature suggests that the use of autoantibodies as biomarkers may enhance early detection, diagnosis and monitoring of the progress of autoimmune disorders^2-5. Autoantibodies can also be used as an adjunct for disease evaluation and to provide a cost-effective personalized approach to guide diagnosis. It can also assist in stratification of patients and in disease prognostication⁶.

ITSI-Biosciences’ unique Autoantibody Detection Kit is specifically developed for detection and quantitation of multiple autoantibodies that may be present in human body fluids such as blood. The kit is for general autoantibody research and autoantibody-wide association studies. Using this you may identify putative autoantibodies that are present in the autoantibody reactome and relevant in a disease process. It can also be used to study autoimmune phenomena attributable to COVID-19 exposure, and the new-onset phenomena after COVID-19 vaccination⁷.

The autoantibody kit includes a set of antigens that detect specific autoantibodies. It is presented in a 96-well microtiter plate format and the kit includes all reagents and materials required for screening, detection and interpretation of the results. The manufacturing process utilizes a unique blocking reagent and protocol that ensures high sensitivity and reproducibility. To increase experimental confidence, each plate has an internal standard for the generation of a standard curve, and a positive and a negative control. The product is easy-to-use, and can be used for qualitative and semi-quantitative assays.

The performance of the autoantibody detection kit has been evaluated using plasma from consented donors with breast and ovarian cancer^8-10.

• KIT CONTENT
  Each Autoantibody detection kit consists of the following:
  • 96-well microtiter plate containing probes for autoantibodies, positive and negative controls, and standard curve.
  • Buffer system.
  • Blocking Reagent.
  • Detection Antibody.
  • Colorimetric Reagent.
  • Quenching Reagent.
  • Color Scoring Card.
  • Protocol.
• APPLICATION OF THE AUTOANTIBODY DETECTION KIT
  The autoantibody detection kit is for research. It is recommended for autoantibody screening, autoantibody discovery, autoantibody validation and autoantibody-wide association studies. The kit can be used for research in the areas such as:
  • Aging
  • Autism
  • Angiogenesis
  • Anti-apoptosis
  • Allergy
  • Cancer
  • Cardiovascular
  • Chronic Obstructive Pulmonary Disease (COPD)
  • Diabetes
  • Rheumatoid Arthritis

Literature Cited

1. https://www.cancer.gov/publications/dictionaries/cancer-terms/def/autoantibody
2. Mol Immunol. 2013 Jan 25;54(3-4):338-346.;
3. Atheroscler Suppl. 2013 Jan;14(1):219-22.;
4. Curr Rheumatol Rep. 2013 Mar;15(3):312.;
5. Atheroscler Suppl. 2013 Jan;14(1):161-5.;
6. Adv Clin Chem, 2024:120:45-67. doi: 10.1016/bs.acc.2024.04.001. Epub 2024 Apr 16.
7. Immunology. 2022;165:386–401. https://doi.org/10.1111/imm.13443
8. Nat. Methods doi:10.1038/an8793, 2013.
9. Cancer Genomics & Proteomics January 2016, 13 (1) 13-19.
10. CANCER GENOMICS & PROTEOMICS 14: 427-435 (2017) doi:10.21873/cgp.20052