Bradford Total Protein Assay Kit (ToPA, K-0014)
$64.50 – $80.16
ToPA is an optimized ready to use kit that simplifies the process of protein concentration measurement. The kit comes in two formats, one for use with cuvettes (ToPA-20) and one for use with 96 well plates (ToPA-96).
Specifications
Applications- SDS-PAGE, Mass Spectrometry, etc.
Sample Type- Protein Lysate
Sample Volume- 2uL
Kit Size- 20 Assays (ToPA-20), 96 well plate Assay (ToPA-96)
Product Details
ITSI’s Total Protein Assay Kit (ToPA) comes in two formats one for use with cuvettes and another for use with 96 well plates. Both kits include all the reagents necessary for the assay as well as a premade standard curve with an r2 of greater than 99 and a full set of cuvettes or a 96 well plate depending on the format purchased
Principle
ToPA is a simple ready to use kit based on the proven Bradford Assay. ToPA has been optimized to be more sensitive than the standard Bradford assay while also being more tolerant to standard concentrations of common buffer systems. With our ready to use reagents and validated procedure you can generate consistent reproducible results with as little as 2ul of sample in under 5 minutes per reading.
Procedure
Using the included Standard Curve Reagents a standard curve is prepared. A small amount of sample is then combined with our Quanti-Protein Assay Reagent (Q-PAR) and the absorbance is measured. The resulting absorbance is then plotted against the standard curve to determine the protein concentration of the sample.
Applications
Protein concentration measurement is commonly the first step for many proteomics processes. ToPA simplifies the process and allows you to move on to the next step of your workflow quickly with consistent reproducible results.
Supporting Data and Figures
Each Kit contains optimized reagents and consumables including:
- Quanti-Protein Assay Reagent
- Ready-to-Use Standard Curve Reagents
- 2000 ug/ml SCR
- Cuvettes or microtitre plates
- 1.5ml Assay Tubes
- Validated lab procedures
Additional information
| Weight | 2 lbs |
|---|---|
| Dimensions | 12 × 9 × 2 in |
| Quantity | 20 Assays, 96 Assays |
Data Sheet(s)
Click on the title(s) below to view the product data sheet.
Citations
Identification of Protein Markers in Patients Infected with Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax.
Alan Kang-Wai Mu, Int J Mol Sci. 2014 Nov 3;15(11):19952-61. doi: 10.3390/ijms151119952.
Article Snippet: “After the sample was concentrated to less than 25 μL, 300 μL of ASM Buffer 2 (provided in the kit) was added to the spin column. The sample was again concentrated to a volume less than 25 μL. The ToPA™ assay kit (ITSI Biosciences, Johnstown, PA, USA) was used to determine protein concentrations.”
The effect of selenium enrichment on baker’s yeast proteome.
K. El-Bayoumy, A. Dasa, S. Russell, S. Wolfe, R. Jordan, K. Renganathan, T.P. Loughranb, R. Somiari. Journal of Proteomics-Elsevier Inc. 2011 October 21;.10.1016
Article Snippet: “After homogenization, the samples were incubated on ice for 40 minutes, while vortexing every 10 minutes, and then centrifuged at 13,000xg for 10 minutes at 4°C. The supernatant was transferred to a new tube and the total protein concentration was determined with the ToPA™ total protein assay kit (ITSI – Biosciences, Johnstown, PA) as previously described [20]. Proteins isolated were used for both comparative proteomics and Western blot analysis.”
Selenium-Responsive Proteins in the Sera of Selenium-Enriched Yeast-Supplemented Healthy American and Caucasian Men.
R. Sinha., I. Sinha., N. Facompreruqui., M. Elmissiry. Journal of Biomedical Science. 2009 September;35(3):559-67.
Article Snippet: “The resuspended sample was incubated on ice for 30 minutes, with four vortexes and centrifuged at 15,000 × g for 10 minutes. The supernatant was transferred to a fresh tube and placed on ice until analyzed on the same day. Total protein content was determined with the ToPA protein assay kit and Bradford protocol (ITSI Biosciences) using bovine serum albumin as a standard.”
Proteomics of rat prostate lobes treated with 2-N-Hydroxylamino-1-methyl-6-phenylimidazo [4,5-b] pyridine, 5alpha-dihydrotestosterone, individually and in combination.
Boyiri, T., Somiari, RI., Russell, S., EL-Bayoumy, K. Int. J Oncol. 2009 Sep;35(3):559-67
Article Snippet: “After homogenization samples were incubated on ice for 30 min, with 4 vortexes, and centrifuged at 16,000 × g for 10 min. The supernatant was transferred to a fresh tube and placed on ice until analysis on the same day. Total protein concentration of each sample was determined with the ToPA™ protein assay kit (ITSI-Biosciences) using the Bradford protocol and bovine serum albumin as standard.”
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