Tech Notes

October 13, 2012 APPLICATION NOTE: PGST & PGPT™ – kit for Plasma and Serum Proteomics to Prevent Ex-vivo Protease Activity

Here we present the Proteomics Grade Tube that streamlines this critical step by inhibiting protease inhibitors at the point of blood collection thereby eliminating the need to transport samples frozen or in a hurry to a laboratory for analysis.

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September 30, 2012 APPLICATION NOTE: ProFEK™ – Kit for isolation of nuclear, cytosol and membrane proteins using a bench top centrifuge

Here we present a kit (ProFEK) and method: that allows researchers to accomplish Sub-cellular fractionation with a bench top centrifuge to identify where proteins are mainly localized and to understand their involvement in a particular disease or signaling pathway.

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August 17, 2012 APPLICATION NOTE: ProDM™ – A kit for tryptic digestion monitoring for a successful shotgun proteomics

This application note illustrates how to: In-solution tryptic digestion of Bovine Serum Albumin (BSA) was monitored with ITSIPrep ProDM kit and SDS-PAGE over a 24hr period to demonstrate the convenience and sensitivity of the approach.

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ITSIPrep Kits for Two Dimensional Difference in Gel Electrophoresis” discusses how to:

1) Achieve great results in 2D-DIGE experiments.

2) Streamline and standardize the up-stream sample preparation steps including protein isolation, fractionation, precipitation and depletion.

3) Avoid the use of poor quality reagents and inadequate protocols which results to poor quality data and waste of time and money.

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