Protein Analysis Using Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE)
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ITSI – Biosciences also offers SDS PAGE, large format Two Dimensional – Difference in Gel Electrophoresis (2D-DIGE) and 1D/2D Western Blotting services. SDS PAGE can be performed with a mini gel or the Agilent BioAnalyzer and biochip. In each case, an automated software program is used to detect, quantify and annotate the protein bands.
We can also screen for phosphoproteins, by staining the 2D-PAGE gels with a phosphor protein stain. The 1D and 2D Gels can also be blotted by western transfer and screened with antibodies supplied by the client.
| S/N | CAT NO. | NAME | DESCRIPTION | UNIT COST |
|---|---|---|---|---|
| 1 | SP1 | Sample Preparation 1 | Homogenization, protein quantitation using ToPA Bradford protein assay kit. | Per sample |
| 2 | SP2 | Sample Preparation 2 | Includes, precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA kits. | Per sample |
| 3 | SP3 | Sample Preparation 3 | Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Column kit. | Per sample |
| 4 | SP4 | Sample Preparation 4 | Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Solvent kit. | Per sample |
| 5 | SP5 | Sample Preparation 5 | Depletion of 14 abundant proteins from serum / plasma using IgY column. | Per sample |
| 6 | SP9 | Sample Preparation 9 | Urine protein isolation and concentration. Service includes Isolation, partial purification, and concentration of urine proteins using ITSI Urine Protein Isolation kit. | Per sample |
| 7 | S10MG | 1D SDS-PAGE – Mini Gel | 1D gel electrophoresis. The service includes • Determining Protein Concentration • SDS-PAGE, • Gel scanning and • Image analysis. | 1-8 samples |
| 8 | S10BA | 1D SDS-PAGE – Bioanalyzer | 1D Gel electrophoresis using Agilent Bioanalyzer and Chip. The service includes • Determining protein concentration • SDS-PAGE with the bioanalyzer, and • Image analysis. | 1-8 samples |
| 9 | S20 | 2D-PAGE | Classical Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE). The service includes • Determining Protein Concentration • 1st dimension, • 2nd dimension, • Staining, • De-staining and • Scanning. | 1 sample Per Gel |
| 10 | S20P | 2D-PAGE PhosPho | Phospho protein profiling. The service includes • Determining Protein Concentration • 1st dimension, • 2nd dimension, • Staining, • De-staining, • Phosphoprotein staining and • Scanning. | Per gel |
| 11 | S101-1D | 1D-Western Blotting | 1D Western analysis using client supplied antibody | Per blot |
| 12 | S101-2D | 2D-Western Blotting | 2D Western analysis using client supplied antibody | Per blot |
| 13 | SP7 | Sample preparation 7 | Manual In-gel digestion. Digestion of proteins in a gel band/spot using trypsin. The service includes • Gel de-staining – coomassie or silver stain • Alkylation and reduction • Trypsin digestion • Peptide extraction from the gels for mass spectrometry analysis. | Per sample |
| 14 | SP10 | Sample preparation 10 | Zip tip cleanup of in-gel or in-solution digested samples…. | Per sample |
| 15 | S50.60 | LC60-MS/MS | Peptide sequencing and protein identification by liquid chromatography – tandem mass spectrometry of pure protein samples. Services include • Tandem mass spectrometry analysis using nano-LC/MS/MS, • Database search and • Identified protein annotation – 60 min run. | Per sample |
| 16 | DBS-PID | Database Search – Protein Identification | Database search for protein identification. The service includes • Downloading the most recent database files from NCBI or other relevant sources • Database search and • Identified protein annotation. | Per sample |
| 17 | DBS-PTM | Database Search – Protein Identification and PTM Mapping | Database search for protein identification and post translational modification mapping. The service includes • Downloading the most recent database files from NCBI or other relevant sources • Database search and • Identified protein annotation. | Per sample |
| 18 | GO | Gene Ontology (GO) Annotation | Annotation of proteins using GO classification for biological process, molecular function and cellular component. | Per sample |