Quantitative multiplexed Protein analysis using the Luminex xMAP technology
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Quantitative Multiplexed RNA Analysis
Most biological and clinical samples can be analyzed, including, tissue homogenate, cell lysate, whole blood, serum, plasma, purified proteins and formalin-fixed paraffin embedded samples. Partial list of assays available: cancer markers, cardiac markers, cellular signaling, cytokines, chemokines, and growth factors, endocrine, isotyping, matrix metalloproteinases, metabolic markers, neurobiology, and transcription factors/nuclear receptors.
Why xMAP technology?
Multiplexing: Up to 100 bioassays per well; including controls.
Parallel analysis: simultaneous quantitation of multiple proteins in the same sample.
Sample amount: less than 50ul for up to 100 data points.
Reproducibility: provides consistent results and allow for easy quality control.
Accuracy: Quantitative results over a 3 to 4 log dynamic range, with strong concordance to ELISA and mass spectrometry.
| S/N | CAT NO. | NAME | DESCRIPTION | UNIT COST |
|---|---|---|---|---|
| 1 | SP1 | Sample Preparation 1 | Homogenization, protein quantitation using ToPA Bradford protein assay kit. | Per sample |
| 2 | SP2 | Sample Preparation 2 | Includes precipitation, buffer exchange, protein quantitation, etc. and the use of ToPI-DIGE, ToPREP and ToPA kits. | Per sample |
| 3 | SP3 | Sample Preparation 3 | Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Column kit. | Per sample |
| 4 | SP4 | Sample Preparation 4 | Depletion of abundant protein e.g. bovine or human serum albumin using ITSI ASK-Solvent kit. | Per sample |
| 5 | SP5 | Sample Preparation 5 | Depletion IgY Column Depletion of 14 abundant proteins from serum / plasma using IgY column. | Per sample |
| 6 | S100XM | xMAP analysis | Quantitative protein analysis using Luminex xMAP technology. The level of up to 100 proteins can be measured simultaneously in 50ul of serum, plasma, tissue lysate and other body fluids. | 1-100 samples |
| 7 | AD100XM | Assay Development for xMAP analysis of Proteins | This service involves development assays to target proteins(s) of interest. | Per target |