In the world of biomedical research, Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples represent one of the richest archives of biological material available. Yet, until recently, extracting meaningful proteomic data from these valuable specimens has been a persistent challenge. That’s where ToPI-F2, ITSI’s Total Protein Isolation Kit for Formalin Fixed Tissue, makes a game-changing impact.
Why FFPE Samples Matter
Every year, pathology labs around the world archive millions of tissue samples in FFPE form. These specimens are often paired with clinical records, making them a powerful resource for retrospective studies, biomarker discovery, and translational cancer research. However, their use in proteomic analysis has been limited due to the challenges of protein extraction from formalin-fixed tissue.
The ToPI-F2 Solution
ToPI-F2 is a complete, ready-to-use kit that enables reliable and reproducible extraction of proteins from FFPE samples, making it ideal for downstream applications like SDS-PAGE, mass spectrometry, and more.
Key Features:
- Sample Compatibility: Optimized for FFPE tissue sections (~5 x 10μ thick sections per assay).
- High Yield: Extracts up to 10,000 μg/mL of protein, depending on tissue type and fixation history.
- Validated Protocol: Includes all necessary reagents and a proven procedure to streamline your workflow.
- Kit Sizes: Available in 10-assay and 20-assay formats to suit labs of different scales.
Proven Performance on Archival Samples
What sets ToPI-F2 apart is its capability to extract proteins from FFPE samples stored for up to 10 years. This opens the door to analyzing vast historical tissue collections, providing a critical link between archived biological material and modern proteomics.
Seamless Integration into Your Workflow
The ToPI-F2 protocol includes a multi-step deparaffinization and incubation process. Once complete, extracted proteins are present in the supernatant, ready for quantification and analysis. Whether you’re running a clinical proteomics project or a comparative study, the straightforward workflow ensures minimal disruption to your lab operations.
Empowering Translational Research
With ToPI-F2, researchers can finally bridge the gap between pathology archives and high-impact proteomic research. Unlock the potential of FFPE tissue for:
- Biomarker identification
- Retrospective cancer studies
- Clinical outcome correlation
- Comparative proteomics
Ready to explore your FFPE archive with confidence?
Discover the ToPI-F2 Total Protein Isolation Kit and bring your samples back to life — proteomically speaking.
Learn More here: https://itsibio.com/product/protein-isolation-kit-for-formalin-fixed-paraffin-embedded-tissue-topi-f2-k-0017/
