Dear Research Scientists,
In the ever-evolving field of medical research, the quest for comprehensive and reliable data is paramount. We are excited to highlight an invaluable resource that can significantly enhance your research endeavors: the vast repository of stored Formalin-Fixed Paraffin-Embedded (FFPE) tissue.
Why FFPE Tissue ?
FFPE tissue are a treasure trove of biological material, meticulously preserved and cataloged over the years. These tissue contain extensive patient data, offering a rich source of information that can be leveraged for a wide range of research applications. From oncology to pathology, the potential uses of FFPE tissue are vast and varied.
Extensive Patient Data
Each FFPE block is not just a sample; it is a gateway to a wealth of patient information. This extensive data can provide insights into patient histories, treatment outcomes, and disease progression. By utilizing these tissue, you can access a comprehensive dataset that can drive your research forward and lead to groundbreaking discoveries.
ToPI-F2 Protein Isolation Kit
The ToPI-F2 kit is able to extract the protein from these FFPE tissues to be used in various downstream applications that use protein extracts.
The ToPI-F2 kit’s ability to extract protein from FFPE tissues opens up a wide range of downstream applications. Here are some examples:
- Western Blotting: This technique is used to detect specific proteins in a sample. The extracted proteins can be separated by gel electrophoresis and then transferred to a membrane for detection using antibodies.
- Mass Spectrometry: This powerful analytical technique allows for the identification and quantification of proteins in complex mixtures. Extracted proteins can be analyzed to determine their composition and abundance.
- Immunohistochemistry (IHC): This method involves staining tissues with antibodies to visualize the presence and localization of specific proteins within the tissue sections. Extracted proteins can be used to validate the results obtained from IHC.
- Enzyme-Linked Immunosorbent Assay (ELISA): This assay is used to quantify the presence of specific proteins in a sample. Extracted proteins can be used as samples in ELISA to measure protein concentrations.
- Protein Microarrays: These are used to study protein interactions and functions on a large scale. Extracted proteins can be spotted onto microarrays to analyze their interactions with other proteins or molecules.
- Functional Assays: Extracted proteins can be used in various functional assays to study their activity, interactions, and effects on biological processes.
These applications are crucial for research in fields such as cancer biology, drug development, and molecular diagnostics.
Maximize Your Research Potential
The stored FFPE tissue represents an untapped resource that can significantly enhance the depth and breadth of your research. By using ToPI-F2 you are able to integrate these tissue into your studies, you can uncover new correlations, validate findings, and contribute to the advancement of medical science.
Tips
- The degree of formalin fixing and the age of the FFPE tissue can influence the amount of protein extracted.
- When downstream applications include mass spectrometry, 2D gel electrophoresis, ELISA or any method that necessitates the removal of SDS, a cleanup step is required. The ITSI Biosciences ToPREP Total protein precipitation kit is recommended for sample cleanup and concentration.
- If additional protein is needed, the procedure can be repeated multiple times, and the final results can be combined.
Real Science, Real Support
At ITSI Biosciences, we are more than a product provider — we are a scientific partner. Our bioanalytical expertise ensures that our kits not only work but are optimized to meet the real-world needs of today’s biomedical researchers.
Need help with your FFPE analysis project? Our team offers consultation and technical support to help you design experiments and interpret results effectively.
Ready to Try the ToPI-F2 Kit? Visit the ToPI-F2 product page, call +1-814-262-7331 or email us at info@itsibio.com for pricing, protocols, or sample requests.
