As the world of molecular biology and biomedical research rapidly evolves, data-rich, high-throughput techniques become increasingly critical for accelerating discovery. ITSI-Biosciences can analyze your samples using Luminex xMAP technology; a powerful tool for multiplexed nucleic acid and protein quantitation. At ITSI-Bio, we perform services to help you get the most comprehensive and accurate results from your research, faster and more efficiently and without having to purchase or maintain costly equipment
What Is xMAP?
The Luminex xMAP® technology combines the principles of flow cytometry with microsphere-based multiplexing, allowing for the simultaneous detection and quantification of up to 500 analytes in a single reaction. Using color-coded beads, each coupled to a specific probe or antibody, Luminex allows researchers to analyze complex biological samples—such as serum, plasma, or cell lysates—with remarkable sensitivity and throughput.
Key Applications of Luminex:
- Cytokine profiling
- Biomarker discovery
- Gene expression analysis
- Pathogen detection
- Pharmacokinetic and toxicological studies
Whether you’re running clinical trials, conducting immunological studies, or monitoring disease states, Luminex provides a scalable solution with high reproducibility and low sample input requirements.
Luminex xMAP vs ELISA
To analyze 38 samples for 48 different cytokines
| ELISA | Luminex xMAP | |
| Plates | 48 | 1 |
| Time | >100 hours | 3 hours |
| Sample Volume | >1ml of serum/plasma
>1ml of cell culture supernatant
|
≤12.5µl of serum/plasma
50µl cell culture supernatant
|
What Is QuantiGene?
QuantiGene® technology (ThermoFisher Scientific) is a hybridization-based assay that enables direct quantification of RNA (and DNA) without the need for reverse transcription or amplification. It relies on branched DNA (bDNA) signal amplification, making it exceptionally robust and accurate—particularly in samples that are difficult to process, such as FFPE tissues or crude lysates.
Unlike traditional qPCR, QuantiGene assays avoid enzymatic steps, reducing variability and improving precision.
Key Benefits of QuantiGene:
- No need for RNA purification or reverse transcription
- Compatible with degraded RNA samples
- Multiplex capability (up to 80 targets)
- Ideal for gene expression profiling, especially in clinical or archival samples
This makes QuantiGene an excellent tool for validating RNA-seq results, monitoring gene expression in drug development pipelines, or conducting toxicogenomic studies. Pre-configured and custom panels are available. Just tell us what you need.
If your project involves cytokine panels or protein biomarkers, our Luminex xMAP service is the go-to. For gene expression studies in challenging sample types, our QuantiGene service offers unmatched reliability and consistency.
Why Choose ITSI-Biosciences?
Our team brings extensive technical expertise and a commitment to scientific rigor. When you partner with us, you gain:
- Tailored assay design: Whether off-the-shelf or custom, we’ll help select the best panel for your needs.
- Comprehensive data analysis: We don’t just run the assays—we help you interpret the results.
- Fast turnaround: We understand your timelines, and we deliver.
- Quality assurance: All services are performed under strict quality control protocols for consistent, publication-ready data.
Let’s Accelerate Your Discovery
From immunology to oncology to toxicology, our mulitplex protein and QuantiGene services can help streamline your workflow and sharpen your insights. Whether you’re validating biomarkers, running clinical research, or developing therapeutics, we’re here to support you every step of the way.
Get in touch today to discuss your project and learn how our multiplexing solutions can drive your research forward.
