GenPlate-neu

GenPlate-neu^® is an automation friendly, high density microtiter plate format-based product for transport and biobanking of blood and other biological fluids. Originally developed with FTA paper, the new generation plates, now called GenPlate-neu contain GenSaver paper. Like the first-generation plates, the GenPlate-neu contains 6-mm disc of paper in each well. GenSaver paper has the same attributes as FTA, but DNA recovery is about 2x more than FTA paper. The technology used in GenPlate-neu has been used to store and protect DNA in dried blood spots since 2006 and is protected by US and International Patents. GenPlate-neu is available in several formats to best fit the storage needs of your samples.

GenPlate-neu Technology

GenPlate-neu is a high-density microtiter plate based method for storing and transporting biosamples at ambient temperatures and the dry state. GeneSaver paper is a macroporous cellulose matrix that is chemically treated to inactivate bacteria and viruses. Upon application of up to 10µl of blood or other biosample directly to the 6mm disc of GeneSaver paper, cells lyse and release DNA. The DNA then becomes entwined in the fibrous network of the cellulose matrix. The entwined biosample is dried completely at room temperature and the dried sample is sealed with an adhesive cover and is ready for storage or transport at ambient temperature.  The chemistry for recovering DNA from GeneSaver paper has been thoroughly validated. DNA stored on and recovered from GeneSaver paper has been reliably used in demanding downstream experiments such as Illumina and Affymetrix microarrays, HLA typing, WGA, PCR, and genotyping and NGS.

Specifications:

• GenPlate-neu is based on a standard 384-well plate size.
• Each well of sample region contains a 6mm disc of GeneSaver paper
• Each disc in the well will hold 10µl of blood, purified genomic DNA (gDNA), purified plasmid DNA (pDNA), crude bacterial suspension (clones), or glycerol bacterial stock
• Each disc yields approximately 100ng of DNA
• Each plate is foil sealed top and bottom
• GenPlate-neu comes in five region patterns (348, 96, 40, 16 and 4)
• Each plate contains a barcode

GenPlate-neu Formats

The most popular format is the 24 region GenPlate-neu that stores biological samples from 24 sources, and each source has 4 replicates. All current sequencing technologies can now do deep whole genome sequencing on the DNA extracted from GenPlate-neu. Any remaining DNA is often put into an ITSIprotect-DNA tube to preserve it for later use as needed. The four replicates ensure that additional samples from the same source are always available when needed.