Quantitative Protein Analysis Using Luminex xMAP Multiplex Protein Assay Technology (S100)
ITSIBIO »High-throughput, low-volume biomarker analysis for discovery and translational research
Our Luminex xMAP–based multiplex protein assay service (S100) enables simultaneous quantification of up to 100 analytes in a single a biological sample. By combining color-coded microsphere technology with dual-laser detection and advanced data analysis, we deliver sensitive, reproducible, and cost-effective multi-analyte profiling to accelerate your biomarker discovery, pathway analysis, and high-content screening.
How The Technology Works
Built on flow cytometry principles, the xMAP platform integrates advanced fluidics, optics, and digital signal processing to perform multiple protein assays within a single reaction.
- Color-coded microspheres
Each bead population carries a unique spectral signature and is coated with a capture reagent specific to a target analyte. - Dual-laser detection
One laser identifies the bead (and therefore the analyte), while a second laser measures reporter fluorescence to quantify the bound target. - Digital data analysis
Specialized software translates bead identity and fluorescence intensity into precise quantitative results for every analyte in the multiplex.
This approach replaces multiple single-plex ELISAs or Western blots with one streamlined, high-content assay. This reduces time, reagent costs and sample consumption.
Platform Capabilities
- Multiplexing capacity: Can detect up to 100 proteins per well
- Low sample volume: As little as 25 µL per reaction
- High sensitivity & specificity: Reliable detection of low-abundance proteins
- High-throughput formats: 96-well plate workflows for large cohort studies
- Broad assay compatibility:
- Proteins and peptides
- Cytokines and chemokines
- Post-translational modifications (e.g., phosphorylation, histone PTMs)
- Receptor-ligand and enzyme interaction studies
Sample Types Supported
- Serum
- Plasma
- Cell culture supernatants
- Cell lysates
- Additional validated matrices upon request
Panels & Custom Assay Development
Pre-configured Panels
- Extensive human and mouse cytokine/chemokine panels
- Disease-focused panels for oncology, inflammation, immunology, metabolic disease, and organ toxicity pathways
- Below is a small example of preconfigured, commercially available panels targeting specific research areas.
Custom Multiplex Panels
We design, optimize, and validate panels tailored to your biomarker targets, sample type, and study goals, whether for exploratory discovery or focused validation.
Applications
- Cytokine and chemokine profiling
- Metabolic syndrome profiling
- Biomarker discovery and validation (oncology, immunology, neurology, cardiometabolic research)
- Multiplexed signaling pathway and phosphorylation studies
- High-content screening and large cohort analysis
Key Benefits
- Reduced sample consumption through multiplexing
- Lower per-sample cost compared to multiple single-plex assays
- Shorter experimental timelines with simultaneous multi-analyte readouts
- High reproducibility across plates and runs
- Scalable workflows for pilot studies through large validation cohorts
- Flexible solutions with off-the-shelf or fully custom panels
Why Partner With Us
- Expertise in panel design, assay optimization, and multiplex troubleshooting
- Industry-standard xMAP instrumentation and validated workflows
- Rigorous quality control and standardized bead chemistries
- End-to-end service: assay setup, high-throughput processing, data analysis, and interpretation
- Clear, comprehensive reporting with consultative support
- Fast turnaround for both discovery and validation studies
Get Started
Maximize throughput while minimizing sample volume and cost.
Contact us to discuss your targets, sample type, and throughput requirements. We’ll recommend the optimal panel, or design a custom multiplex solution, to generate high-quality biomarker data that advances your research.
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