Quantitative RNA and Gene Expression Analysis – QuantiGene™ Plex Assay Services
ITSIBIO »ITSI Biosciences’ QuantiGene™ Plex assay service delivers highly sensitive, reproducible, and multiplexed RNA analysis—without the need for RNA isolation, purification, PCR amplification, or reverse transcription. Built on research and clinically proven branched DNA (bDNA) technology and advanced Luminex flow‑based detection, this quantitative RNA analysis platform provides unparalleled accuracy and throughput for gene expression analysis and biomarker discovery and validation studies.
Cat# S100QGX – Quantigene Analysis of RNA
Cat# AD100QGX – Assay Development for Quantigene Analysis of RNA
Why Choose QuantiGene Plex Technology
Unmatched Multiplexing Power
- Measure up to 80 RNA targets in a single well
- Simultaneous quantitation of multiple RNAs from the same sample
- Single plex assays can quantify individual targets.
Exceptional Sensitivity & Accuracy
- Detect subtle gene expression changes (<10%)
- Quantitative results across a 3–4 log dynamic range
- Strong concordance with microarray and qPCR results
- Superior specificity due to multi‑capture probe design
Targets available
- Preconfigured multiplex panels that target biologically significant pathways and specific diseases.
- It is also possible to develop custom panels or to design a combination of custom targets mixed with predefined panels.
- Single plex targets can be used either predefined or custom.
- Examples of areas of research covered by predefined panels are:
- Apoptosis and Autophagy
- Cancer
- Inflammation and immunology
- Stem cells
- Toxicology
Minimal Sample Requirements
- 80µl or less is needed for up to 80 data points
- Works directly from cell lysates or tissue homogenates
- Compatible with fresh, frozen, FFPE tissue, whole blood, dried blood spots and purified RNA.
Highly Reproducible & Scalable
- Consistent performance ideal for high‑throughput studies
- 96‑ well format
- Automation‑ready workflow for routine screening
How the QuantiGene Plex Assay Works
- Sample Preparation
Cells or tissues are lysed or homogenized to release target RNA—no purification required. - Hybridization
Oligonucleotide probe sets hybridize cooperatively to target RNA and bind to color‑coded Luminex beads. - Signal Amplification
Sequential hybridization of bDNA pre‑amplifier, amplifier, and label probes builds a highly amplified signal. - Detection
Streptavidin‑phycoerythrin generates a fluorescent signal proportional to target abundance.
Signals are read on a Luminex flow cytometer
Applications
Our QuantiGene services support a wide range of research areas, including:
Gene & Nucleic Acid Analysis
- mRNA expression profiling
- miRNA profiling
- DNA copy number variation (CNV)
- Detection of translocations and fusion genes
- Microarray validation
Clinical & Translational Research
- Prospective and retrospective analysis of clinical samples
- FFPE‑compatible workflows
- Small‑volume and low‑abundance sample analysis
Service Features
- Custom assay design for any gene of interest.
- You can provide the panels or let ITSI design the targets for your analysis.
- Access to over 3,700 designed panels and over 22,000+ verified gene targets for pathway and disease‑focused panels.
- Custom multiplex panels and targets can be designed and optimized.
- ITSI provides data analysis.
- Fast turnaround: 2-6 weeks.
- Study design and result interpretation.
- ITSI Biosciences has been in business for over 20 years in the field of assay development and biomarker analysis.
Deliverables
- High‑quality multiplexed gene quantitation data
- Comprehensive data analysis
- Clear, publication‑ready reports
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