At ITSI-Biosciences, we’re proud to offer cutting-edge solutions that empower researchers to go beyond traditional protein identification and dig deeper into dynamic protein expression changes. One of the most powerful tools in our proteomics arsenal is Tandem Mass Tag (TMT) labeling, a robust and versatile approach for quantitative proteomics.
Whether you’re investigating disease biomarkers, tracking protein expression in drug response, or studying signaling pathways, our TMT-based quantitative proteomics service will deliver precise, high-throughput, and multiplexed protein quantitation without the need for gel-based workflows.
What is TMT-Based Quantitative Proteomics?
Tandem Mass Tags (TMT) are isobaric chemical labels used to tag peptides from different protein samples. These tags allow simultaneous identification and quantification of proteins from multiple biological samples in a single mass spectrometry experiment. At ITSI-Biosciences, we offer TMT labeling with 2 to 16 tags, meaning you can compare up to sixteen samples side-by-side in a single LC-MS/MS run. The technique is ideal and powerful for time-course studies, dose response studies, treatment vs. control comparisons, or complex experimental designs.
Why Choose TMT Labeling?
Unlike traditional methods that analyze samples one at a time, TMT enables multiplexing, which saves time, reduces variability, and increases data reliability. With only 5 to 100 µg of protein per sample required, it is also sample-efficient, making the technique perfect for precious clinical or rare biological samples.
Our Comprehensive TMT Service Workflow
We offer a start-to-finish solution designed to deliver high-quality quantitative data you can trust:
- In-Solution Digestion
Proteins are enzymatically digested in-solution into peptides using tried-and-tested protocols to ensure reproducibility and optimal peptide yield. - TMT Labeling (2–16 Tags)
Each sample is labeled with a unique TMT tag. These chemically identical tags fragment to release reporter ions of distinct masses during MS/MS, enabling accurate relative quantification. - SCX Cleanup
Strong Cation Exchange (SCX) chromatography is used to reduce complexity and clean up the sample, improving peptide recovery and MS sensitivity. - Nano-LC-MS/MS Analysis
Peptides are analyzed using high-resolution tandem mass spectrometry coupled with nano-liquid chromatography (nano-LC). This enables deep proteome coverage and sensitive detection of low-abundance proteins. - Database Search & Protein Identification
MS/MS spectra are searched against curated protein databases to identify peptides and corresponding proteins with high confidence. - Full Protein Annotation
Each identified protein is annotated with functional and biological information, giving you insights beyond quantitation. Annotations include pathways, molecular functions, and potential roles in the biological system studied.
Applications of TMT-Based Proteomics
TMT service is ideal for a wide range of applications, including:
- Differential protein expression analysis
- Host cell protein (HCP) profiling
- Biomarker discovery
- Drug response profiling
- Disease mechanism studies
- Time-course experiments
Researchers in oncology, neuroscience, immunology, cardiovascular disease, diabetes, and many other fields have leveraged this technology to make groundbreaking discoveries.
Why Partner with ITSI-Biosciences?
- Expertise You Can Trust: With over 20 years of experience in sample preparation, proteomics and mass spectrometry, our team ensures high-quality results from sample prep to data interpretation.
- Customized Solutions: We work with you during experimental design, and can tailor project to your unique research goals, experimental needs and budget.
- Reproducible Data: The data delivered is reproducible, and the comprehensive annotation and bioinformatics insights will help you move from experimental design to meaningful conclusions faster.
Ready to take your proteomics research to the next level? Contact us today to learn how our TMT-based quantitative proteomics service can support your next big discovery.
📧 Email: info@itsibio.com
📞 Phone: +1-814-262-7331
🌐 Website: www.itsibio.com
