A New Frontier in Non-Invasive Biomarker Discovery
In recent years, urine proteomics has emerged as a promising area of biomedical research. Unlike blood, urine can be collected easily and non-invasively, making it an attractive sample type for discovering biomarkers related to kidney disorders, urinary tract diseases, cardiovascular issues, and even systemic conditions. The ability to analyze proteins excreted in urine offers researchers a window into the body’s molecular processes without invasive procedures.
However, the success of urine-based protein analysis depends heavily on one key step — efficient and reproducible protein isolation. Urine has low protein content and high salt concentration, which makes extraction challenging. This is where ITSI-Biosciences’ ToPI-U Urine Protein Isolation Kit makes a difference.
Introducing the ToPI-U Kit
The ITSIPREP ToPI-U Kit is specifically designed to isolate and concentrate proteins from urine with high yield and reproducibility. Each kit comes with optimized, ready-to-use reagents and consumables that make the process straightforward and reliable — even for researchers new to urine proteomics.
With ToPI-U, you can process up to 20 urine samples, and from just 1 mL of urine, obtain more than 200 μg of total protein. This makes it ideal for applications that demand high-quality protein extracts for multiple downstream analyses.
Designed for Compatibility and Performance
The proteins isolated using the ToPI-U kit are fully compatible with a variety of downstream analytical platforms, including:
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1D and 2D electrophoresis
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2D-DIGE (Two-Dimensional Difference Gel Electrophoresis)
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Western blotting
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Other quantitative proteomic techniques
This versatility allows researchers to move seamlessly from isolation to analysis without worrying about interference from buffer components or contaminants.
In fact, comparative analyses have shown that urine samples processed with ToPI-U yield distinct, reproducible protein profiles for both male and female samples. The clear separation patterns confirm that ToPI-U provides consistent protein recovery suitable for differential expression studies and biomarker validation.
What’s Inside the Kit
Every ToPI-U kit is packaged with all the essentials needed for a complete workflow — no additional reagents or complex preparation steps required. The components include:
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Solution 1, Solution 2, and Solution 3 – Optimized and ready-to-use reagents for efficient protein extraction and cleanup
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5 kDa MWCO concentration device and collection tubes – For concentrating proteins while removing low molecular weight contaminants
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Micro grinder pestles and tubes – To ensure uniform processing and sample integrity
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Capacity: 10 assays per kit
The simplicity of the protocol minimizes hands-on time while maximizing recovery, reproducibility, and purity.
A Validated Method You Can Trust
The ToPI-U kit is not just convenient — it’s validated and proven. ITSI-Biosciences developed and optimized this kit to meet the rigorous demands of proteomic researchers who require reliable, high-quality samples for discovery and clinical applications.
Whether you are studying disease-related biomarkers, exploring urinary proteomes, or developing diagnostic assays, ToPI-U ensures that the foundation of your research — protein isolation — is solid and reproducible.
Why Choose ITSI-Biosciences?
With years of expertise in quantitative proteomics and biomarker discovery, ITSI-Biosciences provides innovative, lab-tested solutions that bridge sample preparation and advanced analytical platforms. Our ToPI series kits, including ToPI-U, are trusted by researchers worldwide for their efficiency, reproducibility, and downstream compatibility.
Start Simplifying Urine Protein Analysis
Unlock the full potential of urine as a diagnostic resource. Choose ITSI’s ToPI-U for fast, reproducible, and high-yield protein isolation — and move your biomarker research forward with confidence.
Email us at itsi@itsibio.com to learn more or purchase the kit here: https://itsibio.com/product/urine-protein-isolation-kit-topi-u-k-0019/
