In proteomics research, precision and reproducibility are paramount. One of the most critical steps in the proteomics workflow is protein identification by mass spectrometry. Typically, proteins are digested into peptides using the enzyme trypsin. Incomplete digestion or enzyme inactivity can significantly affect the quality of data obtained from mass spectrometry (MS), often leading to failed experiments and wasted resources.
To solve this challenge, ITSI-Biosciences has developed an innovative solution: the Protein Digestion Monitoring (ProDM) Kit (K-0021-10) that takes the guess work out of the digestion step.
What Is the ProDM Kit?
The ProDM Kit is the first and only commercially available kit designed to accurately and rapidly monitor the extent of protein digestion without gel electrophoresis. This technology uses a proprietary colorimetric reagent that works with any standard spectrophotometer to quantify digestion efficiency in real time.
Instead of running time-consuming and semi-quantitative SDS-PAGE gels, researchers can now assess digestion quality in a matter of minutes. By ensuring your protein is adequately digested before moving forward, you can significantly improve the success rate of your MS experiments.
Why ProDM Is a Game-Changer for Proteomics
✔️ Eliminates guesswork: Accurately determines the % of protein digested without relying on gel bands or visual interpretation.
✔️ Saves time and resources: Reduces failed mass spectrometry runs due to incomplete digestion or inactive enzymes.
✔️ Cost-effective: Decreases overall cost of proteomics projects by improving sample quality and reducing instrument time waste.
✔️ Easy integration: Works seamlessly with standard lab spectrophotometers—no new hardware or complex software required.
✔️ Fast results: Delivers digestion quality data in a fraction of the time required for traditional methods.
Ideal for Proteomics Core Labs and High-Throughput Workflows
Whether you’re running a high-throughput proteomics pipeline or troubleshooting inconsistent digestion, the ProDM Kit provides the assurance you need to confidently move forward with mass spectrometry. It empowers researchers to validate enzyme activity, optimize digestion conditions, and avoid costly re-analysis.
Get Started with ProDM
If you’re ready to streamline your protein digestion workflow and improve your proteomics outcomes, the ProDM Kit is a must-have tool in your lab. Get in touch with us today.
📧 Email: itsi@itsibio.com
📞 Phone: +1-814-262-7331
Visit www.itsibio.com/prodm to learn more or purchase the kit online. You can also check out our Trypsin Digestion Monitoring Kit here.
