Empowering Precision in Life Science Research
When it comes to research in cancer, neurodegeneration, cardiovascular disease, and other complex conditions, one fact remains constant: accurate quantitation of total protein at the start of any experiment is essential.
At ITSI Biosciences, we understand that even the most sophisticated analytical workflows, from Western blots and ELISA to proteomics and biomarker discovery, depend on accurate and reproducible protein assays. That’s why we developed ToPA (Total Protein Assay) reagent a next-generation protein assay based on the standard Bradford that is helping scientists conduct more reliable and reproducible research inexpensively.
Why Protein Quantitation is Foundational
In cancer and complex disease research, most experiments begin with protein extraction from tissues, cells, plasma, or serum. Protein concentrations must be normalized before labeling or digesting for proteomics, running gels or transferring to membranes, conducting antibody-based assays and comparative and quantitative mass spectrometry. Even though it is well known that a skewed total protein input equals unreliable downstream results, many labs still rely on outdated, buffer-sensitive costly protein assay reagents that vary by protein type, are disrupted by many detergents, reducing agents, or salts and require refrigeration or complicated preparation.
The ToPA Advantage
ToPA is ITSI Biosciences’ improved version of the classic Bradford reagent. It is engineered to meet the demands of modern life science research without breaking the bank:
| Feature | ToPA (Total Protein Assay Reagent) |
|---|---|
| Sensitivity | Detects low protein levels accurately |
| Buffer Tolerance | Compatible with detergents, salts, and reducing agents |
| Convenience | Room temperature stable; includes ready to use standard curve reagent |
| Versatility | Available in tube-based and 96-well plate formats |
| Affordability | One of the most cost-effective protein assay reagents available |
| Validation | Used and trusted by academic and industry labs worldwide |
How ToPA Stacks Up with Classic Bradford
| Feature | Classic Bradford Reagent | ToPA (Improved) |
|---|---|---|
| Sensitivity | Moderate | High |
| Cost Efficiency | Standard | Among the most inexpensive options |
| Buffer Compatibility | Often limited. May interfere | Compatible with most standard buffers |
| Storage & Shipping | Often refrigerated; less stable at RT | Room-temperature stable. Simpler logistics |
| Formats Available | Typically only reagent supplied | Includes standard curve reagent, curvets or 96-well microplate kit |
Supporting Better Disease Research
Numerous research laboratories have validated ToPA in critical biomedical studies. In the selected references cited below, ToPA was used by researchers to measure protein concentration in complex biological samples. The method enabled precise normalization prior to electrophoresis (SDS-PAGE, 2D-PAGE, 2D-DIGE), western analysis and mass spectrometry. ToPA improving the reliability of proteomic data which critical to cancer biomarker discovery.
a) Cancer Epidemiol Biomarkers Prev; 19(9) September 2010
b) Pharmacology & Pharmacy, 2014, 5, 996-1005
c) Int. J. Mol. Sci. 2014, 15, 19952-19961; doi:10.3390/ijms151119952
d) Cancer Genomics & Proteomics 13: 13-20 (2016)
e) Cancer Genomics & Proteomics 14: 427-435 (2017), doi:10.21873/cgp.20052
f) PLOS ONE. https://doi.org/10.1371/journal.pone.0227095 December 30, 2019 24
Accurate Protein Quantitation = Better Research Outcomes
Cancer and complex disease research demand accuracy at every step, starting with protein input. ToPA helps researchers minimize experimental variability, improve reproducibility of quantitative assays and streamline workflows without compromising quality. Whether you’re working on tumor biopsies, plasma proteomics, or cell line models, ToPA provides the precision you need at a price that supports high-throughput screening and routine lab use.
What’s in the ToPA Kit?
Each ToPA kit includes:
- ToPA reagent (ready-to-use)
- Pre-made ready-to-use standard curve reagent
- Simple protocol
- 96-well plate format for high-throughput labs (optional)
No refrigeration. No special handling. No surprises.
Ready to Improve Your Protein Assays?
Whether you’re launching a new cancer biomarker project or standardizing protocols across multiple studies, ToPA is the smart choice.
ITSIBio Tip of the Month
Always normalize your protein samples using a validated assay like ToPA before proceeding to downstream analysis. Remember, small errors at the start can lead to big problems at the end.
ITSIBio News is published monthly by ITSI Biosciences, your trusted source for high-quality, easy-to-use reagents and bioanalytical services. We help researchers do more, accurately, affordably, and efficiently.
Contact us today
Email: itsi@itsibio.com
Phone: +1-814-262-7331
Visit: www.itsibio.com/ToPA
