In any protein analysis workflow, protein precipitation is a critical preparatory step. Whether you’re working on concentration, buffer exchange, or partial purification, the success of downstream applications—such as western blotting, 2D-DIGE, or mass spectrometry—depends on the efficiency and reproducibility of your precipitation method.
Yet, not all precipitation methods are created equal. Inconsistent recovery, costly reagents, and tedious protocols can set back research timelines and inflate budgets. Enter ITSI-Bio’s Total Protein Precipitation Kit (ToPREP), the smarter solution for protein precipitation.
Why Protein Precipitation Matters
When the total protein concentration falls below detection thresholds, precipitation allows you to concentrate the sample to workable levels. It’s also indispensable for removing interfering substances or for buffer exchange—critical in experiments like electrophoresis or immunoassays.
But for precipitation to be effective, the method needs to be reliable, reproducible, and cost-effective, not to mention easy enough to integrate into any lab protocol without requiring extensive troubleshooting.
What Sets ToPREP Apart?
Our Total Protein Precipitation Kit is designed to deliver on all these fronts:
- ✅ ≥95% Protein Recovery – Ensures minimal sample loss, so you don’t compromise on data quality.
- ✅ Lowest Cost per Sample – Ideal for labs running high volumes of experiments.
- ✅ Convenient and Ready-to-Use – No need to source and prep individual reagents.
- ✅ Reproducible Results – Standardized reagents and a validated SOP mean you get consistent outcomes every time.
- ✅ Versatile Application – Suitable for 1D-PAGE, 2D-PAGE, 2D-DIGE, and western blotting.
Each kit includes everything you need:
- ToPREP Buffers 1–3
- Wash Buffer
- ProPreCip Precipitation Booster
- Validated SOP
- Micro Grinder Pestle and Tubes
Designed for Scientists, by Scientists
ToPREP is built with usability in mind. No steep learning curve. No complex instrumentation. Just a straightforward, reproducible protocol that fits seamlessly into your workflow.
Whether you’re processing a single sample or scaling up, the ToPREP Kit is the smart solution for labs that demand quality, consistency, and affordability.
Take the Guesswork Out of Precipitation
Protein precipitation shouldn’t be the bottleneck in your experiment. With our ToPREP Kit, you can streamline sample prep, ensure high-quality recovery, and confidently move on to your analysis phase—knowing your proteins are in top shape.
Ready to optimize your protein prep workflow?
Give us a call or email us at itsi@itsibio.com to learn more or order your kit today → https://itsibio.com/product/total-protein-precipitation-kit-toprep-k-0016/
