Total Protein Precipitation Kit (ToPREP, K-0016)

$520.00

The ToPREP Protein Precipitation Kit is an easy-to-use solution optimized for life sciences, biomedical research, and academic laboratories, enabling efficient protein precipitation from biological samples with greater than 95% recovery. Designed for applications in proteomics, molecular biology, and biochemistry research, the kit includes the ProPreCip protein precipitation booster, fully optimized reagents, and a validated standard operating procedure (SOP) to ensure reproducible, high-quality results across research workflows.

SKU: K-0016 Categories: ,

Protein precipitation is a critical sample preparation step in life sciences, biomedical research, and academic laboratories. It plays a key role in protein concentration when sample levels fall below analytical detection limits, and is widely used for buffer exchange, protein cleanup, and partial purification workflows. For researchers in proteomics, molecular biology, and translational research, an effective protein precipitation method must deliver high recovery, reproducibility, and operational efficiency while remaining cost-effective.

ITSIPrep Protein Precipitation Kits: Optimized for Research Performance

The ITSIPrep platform is designed to meet the rigorous demands of biomedical scientists, university researchers, and core facilities. These kits support consistent, high-yield protein recovery across a range of applications in proteomics research and analytical biochemistry.

Key Benefits:

  • 95% protein recovery for reliable downstream analysis
  • Low cost per sample for routine laboratory workflows
  • Streamlined protocol for fast, convenient processing
  • High reproducibility across experiments and users
  • Ready-to-use reagents to minimize preparation time

ToPREP Protein Precipitation Kit for Proteomics and Molecular Biology

The ToPREP Protein Precipitation Kit is an easy-to-use solution engineered for efficient protein precipitation from biological samples, delivering >90% recovery. Developed for life science research labs and academic institutions, it includes the ProPreCip precipitation booster along with optimized buffers and a validated standard operating procedure (SOP).

This system enables researchers to:

  • Concentrate low-abundance protein samples
  • Perform efficient buffer exchange for downstream compatibility
  • Achieve reproducible protein isolation for sensitive assays

The resulting precipitated proteins are fully compatible with widely used analytical techniques, including:

  • 1D-PAGE and 2D-PAGE
  • 2D-DIGE (Two-Dimensional Difference Gel Electrophoresis)
  • Western blotting and immunodetection workflows

Kit Components (200 Assays)

Each ToPREP kit is designed for high-throughput laboratory environments and includes:

  • ToPREP Buffer 1
  • ToPREP Buffer 2
  • ToPREP Buffer 3
  • ToPREP Wash Buffer
  • ProPreCip Protein Precipitation Booster
  • Validated SOP for total protein precipitation

This protein precipitation solution is ideal for biomedical research labs, academic institutions, and life sciences companies seeking reliable, high-performance sample preparation tools for proteomics and protein analysis.

Additional information

Weight 2 lbs
Dimensions 12 × 9 × 2 in

Data Sheet(s)

Click on the title(s) below to view the product data sheet.

K-0016-10 Total Protein Precipitation Kit

Citations

 

Selenium-Responsive Proteins in the Sera of Selenium-Enriched Yeast-Supplemented Healthy American and Caucasian Men.

R. Sinha., I. Sinha., N. Facompreruqui., M. ElmissiryJournal of Biomedical Science. 2009 September;35(3):559-67.

Article Snippet:  To wash the pellet, 1 mL of chilled buffer-2 (0.2% potassium phosphate monobasic and 0.5% potassium phosphate dibasic) was added followed by 5 μL of ProPreCip (1% proteomics-grade Ficoll; ITSI Biosciences), a precipitation booster.

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